Locus | Primer sequence (5′→3′) | Repeat motif | Allele size range (bp) | Ta (°C) | Fluorescent dye | BLAST to hit description [organism] |
---|---|---|---|---|---|---|
BC1 | F: TACTCCGAAACTCACGCCTT | (CTT)7 | 270–273 | 59 | 6‐FAM |
Nonintrinsic ABC protein 6, putative isoform 2 [Theobroma cacao] |
R: AAAGACGTATCGGTGAAGCG | ||||||
BC5 | F: ACAAAAATGGCGTCTTCTGG | (CAG)7 | 128–134 | 60 | 6‐FAM | — |
R: GCAGGAGATCCATGGTGATT | ||||||
BC9 | F: TTTGAAAGGGAGGGTGTTTG | (GACT)6 | 134–138 | 57 | HEX | — |
R: GAGGAGGAAAAGTTATGTTTTGG | ||||||
BC10 | F: ACCTCCTGCACAGACCATTC | (ACA)8 | 213–216 | 60 | 6‐FAM | — |
R: CATGGGGGAAAATTTTGTTG | ||||||
BC11 | F: TGGGAGCTGAGATTTGATCC | (CAGC)6 | 316–320 | 60 | NED | — |
R: CCCCACTGGATTGATTGATT | ||||||
BC12 | F: TCCATCCAAATTCAGCAACA | (CAG)8 | 147–150 | 60 | HEX |
Auxin efflux facilitator isoform 6 [Theobroma cacao] |
R: GGTTTGCTGCAAGGAGAGTC |