Figure 7.
LGR4 regulates the actin cytoskeleton by modulating the FAK/SRC pathway. A) Actin stress fiber distribution was changed in LGR4 knockdown cells. MDA-MB-231 cells were transfected with control or LGR4 siRNA, then the cells were fixed for actin staining by phalloidin. Scale bar, 20 μm. The experiment was repeated 3 times. B) LGR4 knockdown increased focal adhesion. Control or shLGR4 MDA-MB-231 cells were fixed for focal adhesion plaque staining by vinculin (white arrows: focal adhesions). Scale bars, 20 μm. The experiment was repeated 3 times. C, D) LGR4-deficiency in mouse decreased FAK/SRC/ERK signaling. Western blot of PyMT-Lgr4 CKO and PyMT-Lgr4fl/fl tumors from 12-wk-old mice. C) Representative blot. D) The quantitative histogram represents the means ± sd of 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.