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. 2018 Jan 8;32(5):2658–2675. doi: 10.1096/fj.201700747RR

Figure 5.

Figure 5.

Analysis of differences in antibody production levels by an ELISA and WB. A) The difference in antibody levels (according to an ELISA) between groups rRID and rRID-eGFP relative to the serum samples from rabbits (n = 2) immunized with rRID-eGFP; (lower) WB analysis for the detection of antibodies in the rabbit serum samples. B) Differences in antibody levels between groups rRID and rRID-HAgD relative to the serum samples from rabbits (n = 2) immunized with rRID-HAgD; (lower) WB analysis for the detection of antibodies in the rabbit serum samples. C–F) Differences in antibody levels between groups RID and RID-fused protein relative to the serum samples from mice (n = 3) immunized with an RID-fused immunogen; (lower) WB analysis for the detection of antibodies in the mouse serum samples. G) Differences in antibody levels between groups eLysRS and eLysRS-HAgD relative to the serum samples from mice (n = 5) immunized with eLysRS-HAgD. Error bars indicate the sd of each cohort. The x-axis indicates reciprocal serum dilution (log2). The initial serum dilution value was 1/200 and 1/3200 for mice and rabbits, respectively.