Figure 5.

Angiogenic gene expression remains altered in BPH/5 implantation sites before placentation. A) VEGFa mRNA expression in C57 and BPH/5 implantation sites at E7.5 was quantified using qRT-PCR. mRNA expression of VEGFa was not significantly increased in BPH/5 pregnancies. P = 0.06. B) Representative Western blots of VEGF₁₆₄ and actin in BPH/5 and C57 implantation sites at E7.5. C) Quantification of VEGF₁₆₄ protein expression relative to actin by Western blot analysis. **P = 0.004. D–F) Quantification by qRT-PCR of PlGF, RGC32, and sFlt-1 transcripts in implantation sites at E7.5. D) mRNA expression of PlGF was increased in BPH/5 pregnancies. **P = 0.01. E) mRNA expression of RGC32 was decreased in BPH/5 pregnancies. **P = 0.01. F) mRNA expression of sFlt-1 showed no significant difference between BPH/5 and C57 pregnancies. Results represents the median and IQR; n = 6–10 implantation sites/group.