Figure 4.
YAP/TAZ ablation reduced intrinsic bone failure properties. A) Femora from 8-wk-old Osterix-conditional YAP/TAZ littermates were tested for 3-point bend to failure. B) Representative load-displacement curves collected during testing. C–F) YAP/TAZ deletion reduced extrinsic mechanical properties measured from the load-displacement curves including maximum load (C), stiffness (D), work to maximum load (E), and work to failure (F). G, H) ANCOVA analysis accounting for bone geometry revealed significant differences in intrinsic failure properties (G), but not intrinsic elastic properties (H). Data are presented as individual samples with lines corresponding to the mean and sem (sample sizes: n = 8, except YAPcKO;TAZcKO, n = 1). Repeated significance indicator letters (a, b) signify P > 0.05, while groups with distinct indicators signify P < 0.05 by ANOVA with Bonferroni post hoc test.