Figure 8.

YAP/TAZ-deletion and acute inhibition of YAP/TAZ–TEAD with VP reduced collagen-related gene expression in vivo. Femoral cortical bones from Osterix-conditional YAP/TAZ-deficient mice were harvested to quantify mRNA expression. Expression levels, normalized to GAPDH, were evaluated in Osterix-conditional YAP/TAZ-deficient cortical bone for Col1a1 (A) and SerpinH1 (B). The 16-wk-old WT mice received intraperitoneal injections of VP (100 mg/kg) every 2 d for 2 wk. VP treatment did not significantly reduce mRNA expression levels in bone (C), but differences in CTGF and SerpinH1 were detected in VP-treated livers (D). Repeated significance indicator letters (a, b) signify P > 0.05, while groups with distinct indicators signify P < 0.05 by ANOVA with Bonferroni post hoc test.