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. 2018 Mar 1;123(1):19–27. doi: 10.1080/03009734.2018.1440037

Figure 3.

Figure 3.

(A) Western blot results of expression of AML1/ETO in SKNO-1 cells. The results indicate that expression of the AML1/ETO fusion protein decreased significantly in SKNO-1 cells transfected with siRNA targeting AML1/ETO. SiAGF1 was a siRNA targeting the AML1/ETO fusion gene. SiGL2 was a siRNA targeting P pyralis luciferase, which was used as a negative control siRNA in this study. (B) Heat map of the miRNA array of SKNO-1 cells transfected with the AML1/ETO siRNA and the control siRNA. Levels of 24 differentially expressed miRNAs between the two groups are shown as a heat map. Two paired miRNA samples collected at two independent knockdown experiments were used. (C) Change of expression of four miRNAs in SKNO-1 with and without AML1/ETO knockdown. The results show increased expression of miR-10a, contrasting with decreased expression of miR-129 and miR-130a in SKNO-1 transfected with AML1/ETO siRNA when compared to controls. Standard errors are indicated. (D) Western blot results of expression of AML1/ETO in HeLa cells. The results indicate that AML1/ETO fusion protein was successfully overexpressed in HeLa cells transfected with the AML1/ETO plasmid compared with that transfected with the control vector. (E) Change of expression of four miRNAs in HeLa cells with and without AML1/ETO overexpression. The results show decreased expression of miR-10a, but increased expression of miR-129 and miR-130a in HeLa with AML1/ETO overexpression. Standard errors are indicated. (F, G, H) Expression of miR-10a, miR-100, and miR-129 in AML cell lines. The results demonstrate that these miRNAs are not uniquely upregulated or downregulated in AML cell lines with the t(8;21) translocation (KASUMI-1 and SKNO-1).