Figure 5.

Immunofluorescent staining for complement protein 3 (green) in retinal tissue and ARPE cells; nuclei are counterstained with DAPI. Retinal nuclear layers (ONL, INL, and GCL) are indicated. C3-positive labeling is shown in the ARPE cells (C, E, F) and in the outer retina in co-cultured specimens (C, E). Sparse C3-positive ganglion cells are also seen after 5 days of co-culture (*). C3 labeling in the cultured ARPE cells is cytoplasmic and punctate-like, toward the apical portion of the ARPE cells (indicated by arrows in F). Scale bars, 20 μM.