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. 2018 Feb 13;123(1):28–42. doi: 10.1080/03009734.2018.1431744

Figure 6.

Figure 6.

Immunofluorescent staining for complement protein 4 (green) in retinal tissue and ARPE cells; nuclei are counterstained with DAPI. Retinal nuclear layers (ONL, INL, and GCL) are indicated. C4-positive labeling is seen only in the ARPE cells (C, E, F). C4 labeling in the cultured ARPE cells is cytoplasmic, and C4 shows a more intense expression on the basal part of the ARPE cells (C, E, F) (indicated by arrows in F). Sparse C3-positive ganglion cells are obvious in the cultured control retinal tissue after 3 and 5 days of culture (indicated by * in the GCL). Scale bars, 20 μM.