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. 2018 Feb 13;123(1):28–42. doi: 10.1080/03009734.2018.1431744

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Immunofluorescent staining for calbindin (green) and the soluble terminal complement complex (sC5b-9, red) in retinal tissue and ARPE cells. Retinal nuclear layers (ONL, INL, and GCL) are indicated. Anti-calbindin antibody labels ganglion, horizontal, and amacrine cells in the retinal tissue (A–E). sC5b-9 (indicated by *) labels blood vessels in non-cultured control retinas (A), in retinas cultured for 3 days (B), and in retinas co-cultured for 3 days (C). After retinal culture or co-culture for 3 or 5 days, ganglion cells are sparsely positive for sC5b-9 (indicated by arrows in B–E). In co-cultured specimens, sC5b-9 seems to coincide with calbindin-positive cells in the INL. Scale bars, 20 μM.