Table 2.
Details of antibodies used in Luminex measurements.
| Specificity | Source | Utility | Company | Working dilution/bead |
|---|---|---|---|---|
| C1q | Mouse monoclonal | Capture | Hycult | 3 μg/1.25*106 beads |
| C1q | Mouse monoclonal | Detectiona | Hycult | 1 μg/mL |
| C3c | Rabbit polyclonal | Capture | Dako | 3 μg/1.25*106 beads |
| C3c | Rabbit polyclonal | Detectiona | Dako | 0.5 μg/mL |
| C4BP | Mouse monoclonal | Capture | Bio-Rad | 3 μg/1.25*106 beads |
| C4BP | Mouse monoclonal | Detectiona | Bio-Rad | 1 μg/mL |
| Factor H | Mouse monoclonal | Capture | Hycult | 3 μg/1.25*106 beads |
| Factor H | Mouse monoclonal | Detectiona | Hycult | 4 μg/mL |
| Factor I | Mouse monoclonal | Capture | Abcam | 1.5 μg/1.25*106 beads |
| Factor I | Rabbit polyclonal | Detectiona | Abcam | 3.3 μg/mL |
a
Biotinylated antibody.