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. 2000 Jun;123(2):463–470. doi: 10.1104/pp.123.2.463

Figure 5.

Figure 5

In situ localization of ZeExp1 and ZeExp2 mRNA in zinnia stem tissues. A, Cross-section of the upper stem probed with the antisense (A) and sense (B) ZeExp1 3′-UTR RNA. Arrows indicate some of the punctate patterned hybridization signals. Note that the signals are found exclusively in the vascular bundles (two bundles are highlighted by asterisks) and in cells near where the interfascicular cambium forms. Longitudinal sections of the upper stem were probed with the antisense (C) and sense (D) ZeExp1 3′-UTR RNA and the antisense ZeExp2 3′-UTR RNA (E). Arrows indicate the apical (C) and basal (E) location of the punctate hybridization signals. Note that the indicated flank of cells in (C) and (E) appear to begin tip growth from the apical corners. Longitudinal sections probed with the zinnia Ted3 (F) and 18S rRNA (G) antisense probes, respectively, indicate an even hybridization signal in cells associated with the protoxylem (F) or all cells (G). A and B, Bars = 100 μm; C to G, bars = 50 μm.