Skip to main content
. Author manuscript; available in PMC: 2018 Apr 16.
Published in final edited form as: J Am Chem Soc. 2017 Sep 27;139(40):14098–14108. doi: 10.1021/jacs.7b05168

Figure 3.

Figure 3

(A) Analysis of wild-type IκB-α (lane 1) and the caged IκB-α protein having a bis-o-nitrobenzyl protected phosphate group before (lane 2) and after (lane 3) 3 min of UV irradiation. The samples were analyzed for reactivity to a polyclonal antibody to phosphotyrosine and for 35S-methionine content. (B) Analysis of the caged IκB-α protein having a bis-o-nitrobenzyl protected phosphate group before, and as a function of the time of UV irradiation. Aliquots were taken at predetermined times and analyzed for reactivity to a polyclonal antibody to phosphotyrosine and for 35S-methionine content.