Table 4.
Expression Yields of IκB-α Phosphorylated at Position 42 Using S-30 Preparations Containing Different Modified Ribosomes
| Clone | yield of full length IκB-α, %a
|
||
|---|---|---|---|
| pTyr | Tyr | no activated tRNACUA | |
| wild type | 3 ± 2 | 68 ± 10 | 0.1 ± 0.05 |
| 030405 | 12 ± 2 | 60 ± 4 | 0.8 ± 0.3 |
| 030449 | 20 ± 2 | 93 ± 10 | 0.4 ± 0.2 |
| 030454 | 15 ± 1 | 81 ± 20 | 0.1 ± 0.06 |
| 040412 | 24 ± 3 | 78 ± 6 | 0.15 ± 0.1 |
| 040424 | 5 ± 1 | 28 ± 6 | 0.1 ± 0.05 |
| 030548 | 2 ± 0.8 | 16 ± 3 | 0.2 ± 0.1 |
a
Yield of modified IκB-α was estimated relative to wild-type IκB-α synthesis in the same experiment.