Skip to main content
. Author manuscript; available in PMC: 2018 Apr 16.
Published in final edited form as: J Biomol Screen. 2012 Jun 26;17(10):1348–1361. doi: 10.1177/1087057112451921

Figure 3.

Figure 3

Effect of compounds on the biophysical properties of rat insulin-degrading enzyme (IDE). (A) Thermal melt midpoints (Tm) observed in the presence of IDE activators (100 µM) were determined using a thermal shift assay. Compound-treated rat IDE (0.5 µM) was evaluated across the temperature range 28 to 70 °C. (B) Mobility shifts observed in the presence of IDE activators. The indicated compounds were incubated with 1 mg/mL IDE for 60 min at 37 °C and then analyzed by native polyacrylamide gel electrophoresis (10%). Adenosine triphosphate (A) was used at 3 mM; compounds 1 to 8 were used at 100 µM. D, DMSO. A dashed horizontal line has been drawn across the image at the expected mobility of IDE.