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. Author manuscript; available in PMC: 2018 Apr 16.
Published in final edited form as: J Biomol Screen. 2012 Jun 26;17(10):1348–1361. doi: 10.1177/1087057112451921

Figure 5.

Figure 5

Select compounds enhance in vivo insulin-degrading enzyme (IDE)–dependent production of yeast a-factor. Diluted yeast cultures (1:2000; y272 cotransformed with pWS192 and pWS496) were grown to saturation (72 h) in the presence of activators, and the a-factor produced was recovered and analyzed. Compounds were used at 100 µM, with the exception of compounds 5, 6, and 8 (12.5 µM, 50 µM, and 25 µM, respectively). The raw data (A) were quantified and mean values graphed relative to a DMSO-treated control (B). Each value is normalized to the density of the culture at the time a-factor was collected (n = 4 for all compounds, except 6, for which n = 2); *p < 0.05 and **p < 0.01 relative to the DMSO-treated control. A nearly identical graph is observed in the absence of normalization.