. Author manuscript; available in PMC: 2018 Apr 16.

Published in final edited form as: J Biomol Screen. 2012 Jun 26;17(10):1348–1361. doi: 10.1177/1087057112451921

Table 2.

Kinetic Assessment of Activators

Compound	K_m, µM	V_max, nmol/min/mg
DMSO	183.7 ± 25.94	154.8 ± 12.26
1	107.7 ± 6.42	194.1 ± 5.85
2	117.9 ± 9.04	164.2 ± 6.4
3	122.1 ± 6.08	267.9 ± 6.7
5	148.8 ± 23.67	127.8 ± 10.41
6	92.2 ± 7.7	176.3 ± 7.35
7	148.3 ± 20.07	194.1 ± 13.81
8	123.8 ± 13.14	185.0 ± 9.73

Compounds (100 M final) were evaluated using the fluorescence-based in vitro assay in 0.1 M KPi buffer, pH 7.6. Kinetic parameters were derived using nonlinear regression analysis in GraphPad Prism 4.0.