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. Author manuscript; available in PMC: 2018 Dec 18.
Published in final edited form as: Dev Cell. 2017 Dec 7;43(6):689–703.e5. doi: 10.1016/j.devcel.2017.11.008

Figure 2. Loss of E2F activity affects fatbody function and endocycle progression.

Figure 2

Oil Red (lipid droplets) and DNA (DAPI) (A) and mean ± SD of total tryacylglicerades (TAG)/ protein ratio (B) in WT and dDP mutant larvae (Unpaired T-test, p<0.0001); “**”: binucleate cells. C: EdU labeled replicating regions in dDP−/− fatbody nuclei (WT cells show a non-specific EdU stain of the nucleolus). D: Quantification of mean ± SD of EdU signal/nuclei increase in FB cells (WT: n=53; dDP−/−: n=30; Mann-Whitney test, p<0.0001). dDP−/− nuclei with normal and abnormal shape incorporate BrdU (E, F; mean ± SD of % BrdU positive cells per category; Two-way ANOVA test, p<0.0001), display abnormal shape (E) and mitotic p-H3 (G); compare to WT nuclei. H: Binucleate cells (membrane labeled with F-actin). I: an average of 50% of dDP−/− FB cells are binucleated (plot of mean ± SD of % cells; WT: n=60; dDP−/−: n=151 nuclei).