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. Author manuscript; available in PMC: 2018 May 16.
Published in final edited form as: Cell. 2017 Oct 12;171(5):1110–1124.e18. doi: 10.1016/j.cell.2017.09.039

Figure 5. The cGAS-STING-NLRP3 Inflammasome Can Be Reconstituted in HEK293 Cells.

Figure 5

(A and B) Indicated HEK293T cells were transfected with mm-cGAS or the inactive E211A mutant. Cell viability was quantified by CTB assay and is depicted as mean + SEM of three experiments (A). One immunoblot of two is shown (B).

(C and D) Indicated HEK293 NLRP3 ASC cells were transfected with cGAS or the E211A mutant or stimulated as indicated with or without MCC950. Representative micrographs are shown (C) and were quantified from three experiments (D) and data are depicted as mean + SEM. **p < 0.001, **p < 0.01. Scale bars indicate 50 µm.

See also Figure S4.