REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
AIM2 (D5X7K) Rabbit mAb	Cell Signaling Technology	Cat#12948
anti-Caspase-1 (p20) (human), mAb (Bally-1)	Adipogen International	Cat#AG-20B-0048-C100
anti-Caspase-1 (p20) (mouse), mAb (Casper-1)	Adipogen International	Cat#AG-20B-0042-C100
Anti-CD10 (ALB1)	Beckman Coulter	Cat#IM3633
Anti-CD14 (RMO52)	Beckman Coulter	Cat#PNIM2707U
Anti-CD19 (J3-119)	Beckman Coulter	Cat#A94681
Anti-CD2 (39C1.5)	Beckman Coulter	Cat#A60794
Anti-CD3 (UCHT1)	Beckman Coulter	Cat#A07726
Anti-CD33 (D3HL60.251)	Beckman Coulter	Cat#A54824
Anti-CD34 (581)	Beckman Coulter	Cat#IMI870
Anti-CD4 (RPA-T4)	Beckman Coulter	Cat#A07726
Anti-CD45 (J33)	Beckman Coulter	Cat#B36294
Anti-CD64 (22)	Beckman Coulter	Cat#IM3601U
Anti-CD65 (88H7)	Beckman Coulter	Cat#PNB08164
Anti-CD8 (B9.11)	Beckman Coulter	Cat#A07726
Anti-MLKL (phospho S358) antibody	Abcam	Cat#ab187091
Anti-Viperin Antibody, clone MaP.VIP	Merck Chemicals GmbH	Cat#MABF106
Brilliant Violet 421 anti-human CD163 Antibody	BioLegend	Cat#333611
Caspase-3 Antibody	Cell Signaling Technology	Cat#9662
Caspase-8 (human) monoclonal antibody (12F5)	Enzo Life Sciences	Cat#ALX-804-242-C100
Cathepsin B (D1C7Y) XP® Rabbit mAb	Cell Signaling Technology	Cat#31718
CD11b Monoclonal Antibody (ICRF44), FITC, eBioscience	Thermo Fisher Scientific	Cat#11-0118-42
donkey anti-goat IgG-HRP	Santa Cruz Biotechnology	Cat#sc-2020
F(ab’)2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568	Thermo Fisher Scientific	Cat#A-11019
FITC anti-human CD68 Antibody	BioLegend	Cat#333806
goat anti-mouse IgG-HRP	Santa Cruz Biotechnology	Cat#sc-2005
goat anti-rabbit IgG-HRP	Santa Cruz Biotechnology	Cat#sc-2004
Human IL-1 beta /IL-1F2 Antibody	R&D Systems	Cat#AF-201-NA
Human/Mouse BID Antibody	R&D Systems	Cat#AF860
IRF-3 (D83B9) Rabbit mAb	Cell Signaling Technology	Cat#4302
Mouse IL-1 beta /IL-1F2 Antibody	R&D Systems	Cat#AF-401-NA
MUTED Antibody	Proteintech Group	Cat#24015-1-AP
PE/Cy7 anti-human CD14 Antibody	BioLegend	Cat#301813
Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb	Cell Signaling Technology	Cat#4947
Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb	Cell Signaling Technology	Cat#5483
Purified anti-ASC (TMS-1) Antibody	BioLegend	Cat#653902
STING (D2P2F) Rabbit mAb	Cell Signaling Technology	Cat#13647
TBK1/NAK Antibody	Cell Signaling Technology	Cat#3013
β-Actin Antikörper (C4)	Santa Cruz Biotechnology	Cat#sc-47778
Bacterial and Virus Strains
Francisella tularensis subsp. novicida strain U112	(Meunier et al., 2015)	N/A
Francisella tularensis subsp. novicida strain U112 deltaFPI	(Meunier et al., 2015)	N/A
MVA	(Kastenmüller et al., 2012)	N/A
Chemicals, Peptides, and Recombinant Proteins
2′3′-cGAM(PS)2 (Rp/Sp)	InvivoGen	Cat#tlrl-nacga2srs
2′3′-cGAMP	InvivoGen	Cat#tlrl-nacga23-1
9-Oxo-10(9H)-acridineacetic acid (CMA)	Sigma-Aldrich	Cat#17927-250MG
Bafilomycin A1 from Streptomyces griseus	Sigma-Aldrich	Cat#B1793-10UG
Birinapant	BioVision	Cat#2597-1
Chloroquine diphosphate salt	Sigma-Aldrich	Cat#C6628-25G
CP-456773 sodium salt (MCC950)	Sigma-Aldrich	Cat#PZ0280-25MG
Deoxyribonucleic acid sodium salt from herring testes	Sigma-Aldrich	Cat#D6898-250MG
Doxycycline hyclate	Sigma-Aldrich	Cat#D9891-1G
Dynasore hydrate	Sigma-Aldrich	Cat#D7693-5MG
GeneJuice® Transfection Reagent	Merck Chemicals GmbH	Cat#70967
Hoechst 34580	Thermo Fisher Scientific	Cat#H21486
Hydroxychloroquine sulfate	Sigma-Aldrich	Cat#H0915-5MG
Lipofectamine 2000 Transfection Reagent	Thermo Fisher Scientific	Cat#11668019
LPS-EB Ultrapure	InvivoGen	Cat#tlrl-3pelps
Nigericin sodium salt	Sigma-Aldrich	Cat#N7143-10MG
Pam3CSK4	InvivoGen	Cat#tlrl-pms
Propidium Iodide	Thermo Fisher Scientific	Cat#P1304MP
Recombinant Human IFN-γ	PeproTech	Cat#300-02
Recombinant Human IL-3	PeproTech	Cat#200-03
Recombinant Human M-CSF	PeproTech	Cat#300-25
Recombinant Human TNF-α	PeproTech	Cat#300-01A
Resazurin sodium salt	Sigma-Aldrich	Cat#R7017-5G
Staurosporine (CAS 62996-74-1)	Santa Cruz Biotechnology	Cat#sc-3510A
Thapsigargin	Sigma-Aldrich	Cat#T9033-.5MG
Z-Val-Ala-Asp(OMe)-CH2F [Z-VAD-FMK]	PEPTIDE INSTITUTE	Cat#3188-v
β-Estradiol	Sigma-Aldrich	Cat#E8875-250MG
Critical Commercial Assays
CD14 MicroBeads, human	Miltenyi Biotec	Cat#130-050-201
Human IL-1β ELISA Set II	BD Biosciences	Cat#557953
Human Total IL-18 DuoSet ELISA	R&D Systems	Cat#DY318-05
Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA	R&D Systems	Cat#DY466
CD34 MicroBead Kit UltraPure, human	Miltenyi Biotec	Cat#130-100-453
Human IL1 beta Kit (HTRF)	Cisbio	Cat#62IL1PEC
Human IP-10 ELISA Set	BD Biosciences	Cat#550926
Pierce LDH Cytotoxicity Assay Kit	Thermo Fisher Scientific	Cat#88954
Mouse IL-1 β ELISA Set	BD Biosciences	Cat#559603
MiSeq Reagent Kit v2, 300 Cycles	Illumina	Cat#MS-102-2002
Experimental Models: Cell Lines
BLaER1 human b-cell to monocyte trans-differentiation cell line	(Rapino et al., 2013)	N/A
THP-1	(Schmid-Burgk et al., 2015)	N/A
HEK293T-mmSTING	(Ablasser et al., 2013)	N/A
Immortalized murine macrophages	(Hornung et al., 2008)	N/A
MEF	(Grimm et al., 2014)	N/A
Flp-In T-REx 293 Cell Line	Thermo Fisher Scientific	Cat#R78007
Experimental Models: Organisms/Strains
Nlrp3−/− mice	(Mariathasan et al., 2006)	N/A
Asc−/− mice	(Mariathasan et al., 2004)	N/A
Casp1−/− x Casp11−/− mice	(Kuida et al., 1995)	N/A
Aim2−/− mice	(Jakobs et al., 2015)	N/A
Recombinant DNA
pLenti(p)-EF1-Flag-mm-cGAS	(Gao et al., 2013)	N/A
pLenti(p)-EF1-Flag-mm-cGAS E211A	(Gao et al., 2013)	N/A
pEGFP-hGal3	(Maejima et al., 2013)	Addgene plasmid # 73080
LAMP1-mGFP	(Falcón-Pérez et al., 2005)	Addgene plasmid # 34831
pRP mmSTING mCitrine	This study	N/A
pRP mmSTING	This study	N/A
pLIX mmSTING mCherry	This study	N/A
pRP LAMP1 GFP	This study	N/A
pcDNA5/FRT CMV-tetO2-NLRP3-tagRFP PGK-ASC-mTurq-Flag	This study	N/A
pRP mmSTING mCitrine S366A	This study	N/A
pRP mmSTING mCitrine V155M	This study	N/A
CMV-mCherry-Cas9	(Schmid-Burgk et al., 2014)	N/A
pLK0.1-gRNA-CMV-GFP	(Schmid-Burgk et al., 2014)	N/A
pLIX BLOC1S5	This study
Software and Algorithms
Outknocker	(Schmid-Burgk et al., 2014)	N/A
GraphPad Prism 6	GraphPad	N/A
Cell Profiler	(Carpenter et al., 2006)	N/A
Other
human Brunello CRISPR knockout pooled library	(Doench et al., 2016)	Addgene #73178