Fig. 2.

Suppression of PLD activity in prostate cancer cell lines leads to apoptotic cell death. DU145, PC-3 and LNCaP cells were plated at a density of 50,000 cells/35 mm dish in complete media containing 10% serum. Cells were treated with PLD1 and PLD2 inhibitors (10 μM) for the indicated times. Cells were then harvested and cell viability was measured through trypan blue exclusion as described in Materials and Methods. Cells were harvested and the levels of cleaved PARP were determined by Western blot analysis (DU145 and PC-3 at 24 and 48 hr, LNCaP at 24 hr). (*, p < 0.10; **, p < 0.05, ***, p < 0.01, ****, p < 0.001)