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. 2018 Apr 1;2018:4972410. doi: 10.1155/2018/4972410

Figure 4.

Figure 4

Cisplatin does not sensitize NB cell lines to NK cell recognition. The NB cell lines SH-SY5Y and LA-N-5 were untreated (medium) or treated with 2 μM of cisplatin for 24 hours and used as targets in an NK cell degranulation assay, measured as the CD107a cell-surface expression on human CD3−CD56+CD45+ NK cells from healthy donors. The K562 cell line was used as positive control for NK cell activation. The A549 cell line was untreated or treated with 10 μM of cisplatin for 48 hours and used as positive control for drug effect. (a) A representative experiment out of five performed is shown. The percentage of CD107a+ NK cells is indicated. (b) Summaries of NK cell degranulation of NK cells, isolated by five different healthy donors, following stimulation with untreated and cisplatin-treated cell lines are shown. Dots, percentage of CD107a+ NK cells; horizontal bars, average values. p value compared untreated with cisplatin-treated cell lines (two-tailed paired Student's t-test) (∗∗∗ p < 0.001).