Table 2.
Final distribution of Cel7A activity after hydrolysis of nRPS and alkaline washing using different cellulase mixtures
| Initial activity (IU/mL) | Celluclast | Accellerase | Cellic | |||
|---|---|---|---|---|---|---|
| 7.837 ± 0.341 | 18.107 ± 0.102 | 15.003 ± 0.411 | ||||
| Activity level (IU/mL) | Fraction (%)b | Activity level (IU/mL) | Fraction (%)b | Activity level (IU/mL) | Fraction (%)b | |
| Activity after hydrolysis | ||||||
| Liquid | 4.566 ± 0.508 | 61.3 | 12.646 ± 0.361 | 62.9 | 6.031 ± 0.100 | 40.1 |
| Solid | 2.077 ± 0.121 | 38.7 | 5.391 ± 0.056 | 37.1 | 6.519 ± 0.596 | 59.9 |
| Alkaline washing | ||||||
| Liquid | 1.381 ± 0.077 | 60.2 | 4.281 ± 0.038 | 52.5 | 4.194 ± 0.081 | 41.2 |
| Solid | 0.651 ± 0.071 | 39.8 | 2.791 ± 0.020 | 47.5 | 4.331 ± 0.125 | 58.8 |
| Overall recovery (%)a | 87.9 | 80.8 | 60.2 | |||
Hydrolysis was conducted for 96 h with 18% solids and 20 FPU/gcellulose at 40 °C
aRefers to the sum of the free enzymes on the liquid phase after hydrolysis and alkaline elution
bRefers to the fraction of the total number of IUs found on each fraction