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. 2000 Jun;123(2):521–530. doi: 10.1104/pp.123.2.521

Figure 1.

Figure 1

Purification of recombinant PsCYP15A. A, SDS-PAGE of successive fractions from a nickel affinity column after the addition of elution buffer, pH 4.0; numbers to the side indicate molecular mass of marker proteins (kD); arrowhead indicates recombinant protein band that is the most intense in fraction 5. B, Western blot of electro-eluted recombinant PsCYP15A from fraction 5, using T7 Tag monoclonal antibody. M, Marker lane; R, recombinant protein lane; arrowhead, recombinant protein band; double arrowhead, higher Mr recombinant band, presumably resulting from dimerization of recombinant PsCYP15A monomer.