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. Author manuscript; available in PMC: 2018 Apr 16.
Published in final edited form as: Nat Protoc. 2017 May 24;12(6):1261–1276. doi: 10.1038/nprot.2017.066

Table 4.

Results of amplicon scheme sequencing on five Zika-positive clinical samples from Columbia using the ZikaAsian scheme on the Illumina MiSeq.

Sampl e Ct 1 GE/ul RNA 2 Reads ZIKV reads 3 % ZIKV % Coverage Depth Genbank ID
ZC188 34 36 1,114,568 760,976 68.3 98.3 29,395 KY317936
ZC192 33.9 39 1,246,644 795,474 63.8 98.4 30,396 KY317937
ZC199 35.9 10 2,772,457 379,064 13.7 93.4 14,848 KY317938
ZC204 33.6 48 1,065,517 751,872 70.6 99.7 29,003 KY317939
ZC207 35.2 16 939,820 506,821 53.9 96.7 19,478 KY317940
1

qRT-PCR cycle threshold (Ct) value

2

Genome copy equivalents (GE) per microlitre calculated from Ct value and standard dilutions.

3

Number of reads aligning to a Zika virus reference genome