| Component | Amount (μl) | Final concentration |
| Q5 reaction buffer (5×) | 5 | 1× |
| dNTPs, 10 mM | 0.5 | 200 μM |
| Q5 DNA polymerase | 0.25 | |
| PCR grade water | to 25 | |
| Primer pool 1 or 2 (10 μM) | Varies | 0.015 μM per primer |
| cDNAorDNA | 2.5 |
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