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. Author manuscript; available in PMC: 2018 Apr 16.
Published in final edited form as: Cell Rep. 2018 Feb 20;22(8):1945–1955. doi: 10.1016/j.celrep.2018.01.076

Figure 3. Hyperactive mTORC1 Signaling in the Absence of SIRT3 Contributes to Increased De Novo Purine Synthesis.

Figure 3

(A) Immunoblots of proteins phosphorylated upon mTORC1 activation in WT and SIRT3 KO MEFs after 16 hr of serum starvation or nutrient replete conditions (see also Figures S3A and S3B).

(B) Western blot of WT and SIRT3 KO MEFs treated with 30 μM azaserine for 24 hr.

(C) Glutamine uptake in WT and SIRT3 KO MEFs treated with 100 nM rapamycin or DMSO for 24 hr (n = 6) (see also Figure S3C). Data are represented as mean ± SEM.

(D) Heatmap comparing metabolite levels in the presence of rapamycin (100 nM, 6 hr) in WT and SIRT3 KO MEFs (n = 4) (see also Figures S3D and S3G). Red indicates upregulation and blue indicates downregulation.

(E) Proliferation of WT and SIRT3 KO MEFs treated with 100 nM Torin (see also Figures S3E, S3F, and S3H).

Data are represented as mean ± SD, comparing WT+Torin/WT (black asterisk) and KO+Torin/KO (red asterisk). ***p ≤ 0.001 and ****p ≤ 0.0001.