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. Author manuscript; available in PMC: 2018 Apr 16.
Published in final edited form as: Cell Rep. 2018 Feb 20;22(8):1945–1955. doi: 10.1016/j.celrep.2018.01.076

Figure 4. Incorporation of Glutamine Is Upregulated in SIRT3 KO Cells through the mTORC1.

Figure 4

(A) Schematic of glutamine incorporation into purine and pyrimidine synthesis.

(B) 15N-glutamine tracing into nucleotides in SIRT3 WT and KO MEFs after 8 hr.

(C) Tracing performed as in (B), after treatment with 30 μM azaserine or 100 nM Torin.

(D) Model of SIRT3 regulation of glutamine usage for de novo nucleotide biosynthesis to promote proliferation.

Data in this figure are represented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001.