Table 4. Blackflies and mosquitoes/midges specimens used to test the analytical specificity of qPCR assay.
| Geographical origin | Blackflies species | Number (pos/tot) |
Mosquitoes/ midges species | Number (pos/tot) |
|---|---|---|---|---|
| Greece | Simulium balcanicum | 0/14 | Culex pipiens pipiens | 0/10 |
| Simulium erythrocephalum | 0/6 | Ochlerotatus caspius | 0/10 | |
| Simulium pseudequinum | 0/10 | Anopheles maculipennis | 0/4 | |
| Simulium reptans | 0/23 | Coquilletidia richiardii | 0/2 | |
| Simulium ornatum | 0/4 | Culiseta annulata | 0/3 | |
| Simulium velutium | 0/9 | Culicoides spp. | 0/1 | |
| Ceratopogonidae | 0/5 | |||
| Psychodidae | 0/4 | |||
| Italy | ||||
| Basilicata region | Simulium erythrocephalum | 1/1* | ||
| Simulium linatum | 0/5* | |||
| Simulium ornatum | 1/4* | |||
| Simulium pseudequinum | 0/10* | |||
| Simulium sp. | 6/7* | |||
| Reggio Emilia | Aedes albopictus | 0/20* | ||
| Total | 8/93 | 0/59 |
* = Specimens experimentally infected by intrathoracic microinjection with microfilariae of Onchocerca lupi.