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. 2018 Apr 4;14(4):e1007289. doi: 10.1371/journal.pgen.1007289

Fig 1. The RhoA enhancer activates gene expression in Drosophila abdominal C1-SOPs.

Fig 1

(A, B) Lateral view of Drosophila RhoBAD-LacZ (A) or RhoAAA-LacZ (B) embryos (stage 11) immunostained for β-gal (green) and AbdA (purple). Both reporters are active in a specific cell type (C1-SOP) with higher levels observed in abdominal segments (stained by AbdA, first abdominal segment marked by “A1”) than thoracic segments. (C) The RhoA sequence has binding sites for Pax2, Sens, Exd, Hth, and AbdA that are critical for proper RhoBAD-LacZ and RhoAAA-LacZ activity in Drosophila embryos [17, 22]. (D) Schematic model of competition between activator (Pax2/Exd/Hth/AbdA) and repressor (Sens) TFs. Sens binds and represses RhoA activity in the thorax; whereas AbdA and the activators outcompete Sens to promote gene activation in C1-SOP cells of the abdomen.