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. 2018 Mar 14;7:e34282. doi: 10.7554/eLife.34282

Figure 4. The effect of consubspecific PWD/PWD stretches of genomic sequence on pachytene synapsis and meiotic progression, 4-chr cross.

(A) Scheme of a four-generation cross resulting in F1 hybrids with four recombinant consomic chromosomes. (B) The asynapsis rate related to the size and chromosomal position of the consubspecific PWD/PWD sequence in four consomic chromosomes (15, 16, 18 and 19, see also Figure 4—source data 3). The localization of PWD homozygous sequence with respect to the centromere, the interstitial part of the chromosome, or the telomere, or on both ends is distinguished by color (see also Figure 4—source data 3). (C) Number of sperm in epididymis is a function of the probability of synapsis of all four consomic chromosomes. The complete meiotic arrest is reversed in males having 70% or higher chance of all four chromosomes synapsed. See Figure 6—figure supplement 2. Loess curve with 95% CI.

Figure 4—source data 1. Eleven G3 male parents selected for the 4-Chr cross experiment.
elife-34282-fig4-data1.xlsx (11.4KB, xlsx)
DOI: 10.7554/eLife.34282.019
Figure 4—source data 2. The fertility parameters of hybrids of the 4-chr cross experiment.
elife-34282-fig4-data2.xlsx (11.3KB, xlsx)
DOI: 10.7554/eLife.34282.020
Figure 4—source data 3. Four-chr cross.
The effect of the size and location of PWD/PWD consubspecific intervals on asynapsis of Chrs 15, 16, 18 and 19 and on fertility parameters.
elife-34282-fig4-data3.xlsx (19.3KB, xlsx)
DOI: 10.7554/eLife.34282.021

Figure 4.

Figure 4—figure supplement 1. Fertility parameters of G4 males from the 4-chr cross.

Figure 4—figure supplement 1.

Rescue of HS meiotic arrest is detectable in males with the weight of paired testes > 70 mg and >0.1×106 of sperm in the epididymis. The males selected for DNA FISH/HORMAD2 analysis of asynapsis are highlighted in turquoise. All males share the sterility-determining allelic combination of Prdm9PWD/B6 and Hstx2PWD. Loess curve with 95% CI.