Skip to main content
. 2018 Mar 27;7:e32581. doi: 10.7554/eLife.32581

Figure 1. L. reuteri is inhibited by 18:2.

(A) Disc diffusion of L. reuteri plated with the FAs of SBO. FAs were dissolved in DMSO to a concentration of 50 mg/ml, except for palmitic acid (16:0), which was dissolved to a concentration of 5 mg/ml. Clearings around the discs indicate growth inhibition. (B) Dose response curve of 18:2 with L. reuteri. IC50 is estimated at 20 μg/ml (p < 0.001). See also Figure 1—figure supplement 1.

Figure 1.

Figure 1—figure supplement 1. L. reuteri is inhibited by the hydrolysis products of SBO.

Figure 1—figure supplement 1.

Disc diffusion assay of L. reuteri plated with each SBO FA and glycerol mixed to match their composition in soybean oil (FA mix + glycerol). Disc diffusions with 16:0, 18:2, total soybean oil, DMSO and glycerol, and a mix of only the FAs in SBO (FA mix) are shown as controls. Note that the size of a zone of inhibition reflects both the toxicity of the compound as well as the migration of the compound off the disc: the saturated FAs in the FA mix deter migration whereas the unsaturated FAs move well off the disc.