Figure 2.
Protocols of Exp. 1–3 (A) and Exp. 4 (B). A, Mice were ovariectomized (OVX) and one week later received s.c. capsules containing 180 µg/mL E2 or vehicle. Circadian rhythms of TCORE, TSKIN and activity were recorded over a 5 day period with mice in their home cages. From days 13–21, the mice were exposed to various TAMBIENT in an environmental chamber. The next day, mice were injected s.c. with either senktide or vehicle. Mice received a second injection two days later in a crossover design. B, Protocol of Exp. 4. Ten mice were OVX and 15 days later implanted with s.c. capsules containing 360 µg/mL E2. The capsules were removed after 7 days and after a wash out period, the mice were implanted with capsules containing 720 µg/mL E2. Circadian rhythms of TCORE, TSKIN and activity were recorded for 3 day intervals in the animals home cages (grey bars). Black arrowheads, OVX; grey arrowheads, capsule implants; open arrowheads, blood sample collection. E2, 17β-estradiol; OVX, ovariectomized; VEH, vehicle.