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. 2018 Feb 22;19(4):260–270. doi: 10.1080/15384047.2016.1250981

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 5. — miR-326 and curcumin combination treatment decreased the stemness ability and expression of p53 in glioma cells. (A) U251 cells with or without transfection of miR-326 and/or curcumin treatment were analyzed with immunofluorescent staining using anti-CD133 and anti-Nestin antibodies. Bars represent 20 μm. (B and C) U87 cells with or without transfection of miR-326 and/or curcumin treatment were harvested after 0.5, 1, 3, and 6 h and subjected to RT-PCR and western blot assay to determine p53 expression.