Figure 5. A295S Actin Impairs Drosophila Flight Kinematics.

(A) Representative flight trajectories, and instantaneous acceleration values at every sampled point, for w¹¹¹⁸, heterozygous Act88F^WT/+, and heterozygous Act88F^A295/+. Act88F^A295S/+ mutants are characterized by low acceleration values over the length of their flight paths.

(B) Probability distribution plots of velocity, angular velocity, acceleration, and centripetal acceleration for w¹¹¹⁸ (gray, n = 80), Act88F^WT/+ (black, n = 90), and Act88F^A295S/+ (red, n = 30) flies. While no difference in mean velocity or angular velocity was detected between Act88F^WT/+ and Act88F^A295S/+, both were significantly lower for each line relative to w¹¹¹⁸. Moreover, no difference in acceleration during ascending flight was observed for w¹¹¹⁸ versus Act88F^WT/+, while acceleration for the mutant line was significantly lower than that for both. Similarly, centripetal acceleration for Act88F^A295S/+ was significantly lower than that for w¹¹¹⁸ and Act88F^WT/+.

See also Figures S4 and S5.