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. 2018 Jan 20;6(2):160–170. doi: 10.1002/mgg3.342

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© 2017 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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CBS mRNA expression as determined by qRT‐PCR. Genotypes: P1 (c.209+1delG; c.572C>T); P2 (c.2T>C; c.828+1G>A); P3 (c.828+1G>A; c.1126G>A); P4 (c.833T>C; c.989_991delAGG); P5 (c.1058C>T; c.146C>T); and P6 (c.444delG; c.444delG). Fold change in gene expression is calculated through the 2^−∆∆ ^C_T method, where ∆C_T = (C_T _{,Time x} ‐ C_T _{,Time 0}) (Schmittgen & Livak, 2008)