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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: J Autoimmun. 2018 Jan 4;89:171–185. doi: 10.1016/j.jaut.2017.12.017

Figure 9. Inhibition of desmosomal cadherin-mediated bead aggregation by FS antibodies.

Figure 9

(A) Co-aggregation of cadherin-coated red and green fluorescent beads mixed in homophilic (Dsg1/Dsg1, Dsg3/Dsg3, Dsc1/Dsc1) or heterophilic combinations (Dsg1/Dsc1 and Dsg3/Dsc1). (B) Inhibition experiments showing heterophilic aggregation of green Dsc1-coated beads with red Dsg1 (top panels) or Dsg3- coated beads (bottom panels) pre-incubated with purified IgG4 fractions from FS patients or from a healthy donor (see methods for details). (C) Dose-dependence of IgG4 Fab mediated inhibition of Dsg1/Dsc1 bead aggregation from donors FS 1 and FS17. Scale bar 0.5mm.