. Author manuscript; available in PMC: 2019 May 1.

Published in final edited form as: Wiley Interdiscip Rev Syst Biol Med. 2018 Jan 15;10(3):e1415. doi: 10.1002/wsbm.1415

TABLE 2.

Split luciferases.

Organism	Species	Luciferase name	Length (amino acids)	Size kDa	Split name	N-terminal split	Split name	C-terminal split	Substrate
Firefly	Photinus pyralis	Firefly Luciferase	550	61	NLuc	2–416	CLuc	398–550	D-luciferin
Click beetle	Pyrophorus plagiophthalamus	Click Beetle Red	542	64	CBR (1–414)	1–414	CBR (395–542)	395–542	D-luciferin
Click beetle	Pyrearinus termitilluminans	Emerald Luciferase	542	64	ELucN	1–415	ELucC	394–542	D-luciferin
Sea pansy	Renilla reniformis	Renilla Luciferase	311	36	N-Rluc	1–229	C-Rluc	230–311	Coelenterazine
Copepod	Gaussia princeps	Gaussia Luciferase	185	20	Gluc(1)	1–93	Gluc(2)	94–185	Coelenterazine
Shrimp	Oplophorus gracilirostris	Nanoluc	171	19	N65	1–65	66C	66–171	Furimazine
Shrimp	Oplophorus gracilirostris	NanoBiT	171	19	11S	1–156	114	158–168	Furimazine