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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Dev Dyn. 2018 Mar 23;247(5):741–753. doi: 10.1002/dvdy.24624

Figure 3.

Figure 3

Lymphatics develop at postnatal stages and extend along meningeal blood vessels from the side of the skull towards the midline. (A, D) At P6, lymphatics are not observed in the meninges at the top of the skull. Prox1-GFP-negative/LYVE-1-positive tissue-localized macrophages had invaded the meninges and are mainly associated with the sinuses. (B, E) At P13, Prox1-GFP/LYVE-1-positive lymphatics are visible along the TS at top of the skull (arrow). (C, F) By P16, lymphatics extending along the TS reach the SSS (C, arrowhead) through the COS (C, arrow). The boxed regions in (A–C) are magnified in (D–F). The boxed region in (F) is magnified in (G–H), with staining using different antibodies. (G–G′) Near the TS at P16, developing Prox1-GFP-positive vessels (arrows) and cell clusters (arrowheads) are stained by Prox1 antibody staining. Note that there is some non-specific Prox1 antibody staining (open arrows). (H-H′) Near the TS at P16, extending Prox1-GFP-positive vessels (arrows) and cell clusters are also Podoplanin-positive. (I-I″) Lymphatic vessels in P6 ear skin are already established (I-I″, arrows), indicating that meningeal lymphatic vessels develop at a delayed developmental time point. Open arrowheads indicate LYVE-1-positive tissue-localized macrophages. Scale = 1mm in (A–C) and 100 μm in (G–I, G′-I″).