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. 2018 Apr 16;8:6019. doi: 10.1038/s41598-018-24486-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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C_T values for ten candidate reference genes in R. opacus. RNA was extracted from biological triplicates of R. opacus grown in four distinct growth conditions (HN [circle], LN [triangle], TSB [diamond], and PHE [square]; see Materials and Methods for full description). RT-qPCR was performed in technical triplicate on each biological replicate. Each point represents the average threshold cycle (C_T) value of all replicates for the listed gene and growth condition. Error bars represent one standard deviation.