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. 2018 Apr 10;9:674. doi: 10.3389/fmicb.2018.00674

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Copyright © 2018 Lourenço, Cassman, Pijl, van Veen, Cantarella and Kuramae.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neighbor-joining tree of the 30 most abundant sample amoA-AOB OTUs and their nearest neighbors in the custom FunGene amoA sequence database. The NCBI taxonomic classification of the database entries is included. The outgroup is Nitrosococcus oceani. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches (black points mean bootstrap value >75%). Evolutionary distances were computed using the Maximum Composite Likelihood method. The analysis involved 131 sequences and 305 positions and was conducted in MEGA7.