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. 2000 Jun;123(2):575–588. doi: 10.1104/pp.123.2.575

Figure 3.

Figure 3

Seedlings of EMS treated RLD Arabidopsis during the screening and selection process. A, Putative acquired thermotolerance mutant challenged at 44°C for 30 min identified by reduced chlorophyll accumulation (white circle) despite a 38°C for 4 h pre-incubation and a 16-h light exposure at 25°C. B, Chlorophyll accumulation in the putative acquired thermotolerance mutant shown in A. C, D, and E, A Putative acquired thermotolerance mutant (left) and a thermotolerant seedling (right) after 24, 48, and 72 h of continuous light respectively. F, G, and H, A Pigment mutant (top) and a thermotolerant seedling (bottom) after 24, 48, and 72 h of continuous light respectively. I, RLD seedlings pre-incubated at 38°C for 4 h and challenged at 46°C for 30 min. J, AtTS02 seedlings pre-incubated at 38°C for 4 h and challenged at 46°C for 30 min. RLD (K) and AtTSO2 (L) seedlings pre-incubated at 38°C for 4 h, challenged at 46°C for 30 min, and allowed to accumulate chlorophyll for 6 d. M, Light-grown RLD and AtTS02 seedlings 48 h after a 4-h, 38°C pre-incubation and 30-min challenge at 46°C, 48°C, 50°C, or 52°C.