FIGURE 5.

(A) Immunofluorescent images of lung microcirculation stained with glycocalyx marker – lectin I (labeled by Cy3, red channel) in endo-αENaC^KO and control mice in basal conditions (saline) and in endotoxemia. (B) Quantification of immunofluorescent staining for lectin I. Ten randomly chosen eyefields near the regions of microcirculation were photographed for each mouse and subjected to quantification of relative immunopositive area to the all tissue area with the Columbus software, control (saline) n = 7, endo-αENaC^KO (saline) n = 7, control (LPS) n = 8, endo-αENaC^KO (LPS) n = 8. Statistics: Kruskal–Wallis test followed by Dunn’s post hoc test (normality was assessed using the Kolmogorov–Smirnov test). The results are presented as the median with interquartile range, ^∗p < 0.05, ^∗∗∗p < 0.001