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. 2018 Apr 10;9:712. doi: 10.3389/fmicb.2018.00712

FIGURE 1.

FIGURE 1

Trichomonas vaginalis induces cytokines secretion in a TLR2-dependent way in mouse peritoneal macrophages. RT-qPCR analysis for TLR2 in total RNA isolated from mouse peritoneal macrophages incubated in medium alone, with T. vaginalis or Pam3CSK4 (10 μg/ml), respectively. RT-qPCR demonstrated that T. vaginalis stimulation significantly enhances TLR2 gene expression in WT macrophages (A). WT macrophages were co-incubated with T. vaginalis or Pam3CSK4 for 2 h (B). Relative Gray analysis of western blot (C). WT and TLR2-/- mouse peritoneal macrophages were co-incubated with T. vaginalis. The levels of IL-6, TNF-α, and IFN-γ in cell culture supernatant were detected by ELISA. Compared with TLR2-/- mouse peritoneal macrophages, the production of IL-6, TNF-α, and IFN-γ in WT macrophages were significantly increased (D–F). Data are expressed as the mean ± SD from three separate experiments (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).