Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 16;9:1488. doi: 10.1038/s41467-018-03943-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — BL001 promotes beta and alpha cell mass expansion in immunized RIP-B7.1 mice. The control (C) mass of beta (a, b) and alpha cells (c, d) of RIP-B7.1 mice was decreased after immunization (IMN), an alteration that was prevented by 10 mg/kg b.w. BL001 daily treatment for 8 weeks (BL IMN). Dot plots show the results from 8 (4 weeks) and 16 (8 weeks) pancreas per group, each dot corresponding to the cell mass of an entire pancreatic section. e–j Representative immunofluorescence images of islets co-stained and quantified for e, f insulin (INS, red) and Ki67 (green), g, h insulin (red) and Tunel (green), i, j glucagon (GLUC, red) and Ki67 (green). All values are means of 5 mice per group. Scale bars: 25 µM. Right panels are enlargements of boxed area, and arrow heads point to example of cells simultaneously stained for 2 proteins. k Representative confocal images showing the distribution of the insulin- (red) and glucagon-bihormonal cells (green) as well as l quantification in islets of the different animal groups. Nuclei were stained with DAPI (blue). Scale bar: 25 µM. Right panel show enlargements of boxed areas. Arrow heads point to cells co-stained for the two depicted markers. m A representative image depicting co-immunostaining of PDX1 (red) and glucagon (green) in a pancreas of a normoglycemic immunized BL001-treated mouse, and n quantification of such cells in the various groups. Bottom left panels are enlargements of boxed area. Bottom right panels, are without DAPI staining. White arrows point to PDX1⁺/GLUC⁺ cells, # identifies a PDX1^-/GLUC⁺ cell and * highlights a PDX1⁺/GLUC^- cell. n = 15 pancreatic slices obtained from five independent mice per group. Scale bar: 25 µM. o After a 24-h exposure to 10 μM BL001 (BL), alpha TC-1.6 cells featured decreased expression of the Arx, glucagon (Ggc) and MafB transcripts. Six independent experiments were performed and subsequent QT-PCR conducted in triplicates. Values are means + s.e.m. (f, h, j, l, n, o). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, one-way ANOVA (a–d, f, h, j, l, n) and the Student’s t test as compared to control (o)