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. 2018 Apr 10;9:411. doi: 10.3389/fpls.2018.00411

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Copyright © 2018 Qian, Zhao, Du, Zhao, Han and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NbHsp90 Interacts with Tm-2² in Plant Cells. (A) NbHsp90 co-immunoprecipitated (Co-IP) with Tm-2². HA-NbHsp90 was transiently expressed with Tm-2²-myc in Nicotiana benthamiana leaves by agroinfiltration. HA-NbHsp90 co-expressed with cLUC-myc was used as negative control. At 48 h post infiltration (hpi), total protein extracts were immunoprecipitated (IP) with anti-HA beads and the resulting precipitates were assessed by western blotting using anti-HA antibodies (upper panel, left) and anti-myc antibodies (lower panel, left). The presence of HA-NbHsp90, Tm-2²-myc and cLUC-myc in the immunoprecipitates and cell lysates were also analyzed (right). IB, Immunoblot. (B) Firefly luciferase complementation imaging (LCI) assays for the in vivo interaction of NbHsp90 with Tm-2². cLUC-NbHsp90 was transiently co-expressed with Tm-2²-nLUC or nLUC in N. benthamiana leaves followed by LCI assay.