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. 2018 Apr 10;9:411. doi: 10.3389/fpls.2018.00411

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Copyright © 2018 Qian, Zhao, Du, Zhao, Han and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NbHsp90 Interacts with NbSGT1 in Yeast and in Plant Cells. (A) Yeast cells harboring NLS-LexA BD-SGT1 baits transformed with AD-NbHsp90 grew on Leu^- deficient medium and turned blue on X-gal medium containing galactose (Gal) and raffinose (Raf) but not on medium containing glucose (Glu) at 28°C for 4 days. Yeast cells transformed with either AD or BD empty vector alone were used as negative control. (B) Firefly LCI assays for the in vivo interaction between NbHsp90 with SGT1. cLUC-NbHsp90 was transiently co-expressed with SGT1-nLUC or nLUC in N. benthamiana leaves followed by LCI assay.