FIG 3.

Diminished TCF4/β-catenin transactivation activity in IDH1-MT cells. (A) Coimmunoprecipitation assays with anti-β-catenin antibody show diminished formation of the nuclear β-catenin–PKM2–TCF4 complex in cells overexpressing IDH1-R132H compared to that in cells overexpressing IDH1-WT. IB, immunoblotting. (B) TCF4 transactivation activity in glioma cells was determined by a luciferase reporter assay using TOP Flash and FOP Flash (TOP Flash mutant). The graph depicts TOP/FOP Flash fold changes in relative luciferase units with respect to the control. Values represent the means ± standard errors of the means from three independent experiments. P values were determined by a 2-tailed, paired t test using GraphPad Prism. *, P < 0.05; **, P < 0.01.