Skip to main content
. 2018 Apr 16;38(9):e00047-18. doi: 10.1128/MCB.00047-18

FIG 6.

FIG 6

Loss of a functional Asp1 pyrophosphatase domain results in increased IP8 levels. (A) Diagrammatic representation of Asp1 variants analyzed. All variants were expressed from the endogenous asp1+ locus. (B and C) HPLC elution profiles of inositol polyphosphates of the wild-type type (WT) and asp1H397A strains. (D) Comparison of part of the inositol pyrophosphate profiles of the wild-type and asp1H397A strains. (E) Diagrammatic representation of IP8 levels relative to those of IP7 (WT, n = 4; asp1H397A strain, n = 3; *, P ≤ 0.05, t test). The fold change of IP8/IP7 is 2.81 for the asp1H397A strain relative to the level of the wild-type strain. (F) HPLC elution profile of inositol polyphosphates of the asp11–364 strain. (G) Comparison of inositol pyrophosphate profiles of the wild-type and asp11–364 strains (data used for this wild-type strain were obtained from a strain grown in parallel to the asp11–364 strain). (H) Diagrammatic representation of IP8 levels relative to those of IP7 and normalized to the wild-type level (WT, n = 4; asp11–364 strain, n = 3; **, P ≤ 0.01, t test). The fold change of IP8/IP7 is 1.67 for the asp11–364 strain relative to the level of the wild-type strain.