TABLE 1.
Strains used in this study
| Strain | Genotypea | Source |
|---|---|---|
| S. pombe strains | ||
| UFY605 | his3-D1 ade6-M210 leu1-32 ura4-D18 h− | K. Gould |
| UFY1156 | asp1Δ::Kanr his3-D1 ade6-M216 leu1-32 ura4-D18 h− | U. Fleig |
| UFY1511 | asp1D333A::Kanr his3-D1 ade6-M210 leu1-32 ura4-D18 h+ | U. Fleig |
| UFY1565 | cdc11-123 leu− h− | Yeast Genetic Research Center Osaka, Japan (FY8347)b |
| UFY1579 | asp1H397A::Kanr his3-D1 ade6-M210 leu1-32 ura4-D18 h+ | U. Fleig |
| UFY1687 | cut7-GFP::Kanr cut12-CFP::Natr leu1 ura4 h− | Yeast Genetic Research Center Osaka, Japan (FY17673) |
| UFY2257 | bub3Δ::Kanr leu1− h− | Yeast Genetic Research Center Osaka, Japan (FY18583) |
| UFY2290 | bub3Δ::Kanr asp1D333A::Kanr | U. Fleig |
| UFY2294 | asp11−364::Kanr ura4-D18 leu1-32 his3-D1 ade6-M21x h+ | This study |
| UFY2386 | bub3Δ::Kanr asp1D333A::Kanr sad1-mCherry::Kanr LacI-GFP::his7+ LacO-repeat::lys1+ lys1-131 his7-366 h− | U. Fleig |
| UFY2553 | asp1I808D::Kanr his3-D1 ade6-M210 leu1-32 ura4-D18 h− | This study |
| UFY2758 | met10Δ::Kanr his3-D1 ade6-M210 leu1-32 ura4-D18 h− | This study |
| UFY2795 | met10-GFP::Kanr his3-D1 ade6-M210 leu1-32 ura4-D18 h− | This study |
| UFY2805 | met10Δ::Kanr asp1D333A::Kanr his3-D1 ade6-M210 leu1-32 ura4-D18 h− | This study |
| UFY2807 | met10Δ::Kanr asp1H397A::Kanr his3-D1 ade6-M210 leu1-32 ura4-D18 h− | This study |
| UFY2860 | met10Δ::Kanr mal3Δ::his3 ade6-M210 leu1-32 ura4-D18 his3-D h+ | This study |
| UFY2940 | asp1D333A-GFP::ura4+ cox4-RFP::LEU2 ade6-M210 leu1-32 ura4-D18 his3-D1 h− | This study |
| UFY2941 | asp1-pk-GFP::ura4+ cox4-RFP::LEU2 ade6-M21x leu1-32 ura4-D18 his3-D1 h+ | This study |
| UFY2951 | asp1Δ::Kanr cox4-RFP::LEU2 ura4-D18 leu1-32 his3-D1 ade6-M21x h− | This study |
| UFY3035 | cut7-GFP::Kanr leu1 ura4 his3-D1 | This study |
| UFY3039 | asp1D333A::Kanr cut7-GFP::Kanr his3-D1 ade6− leu1 ura4 | This study |
| S. cerevisiae strains | ||
| AH109 | MATα ura3-52 trp1-901 leu2-3112 his3-200 gal4Δ gal80Δ LYS2::GAL1UAS-GAL1TATA-HIS3 GAL2UAS-GAL2TATA-ADE2 URA3::MEL1UAS-MEL1TATA-lacZ | Clontech |
| Y187 | MATα ura3-52 trp1-901 leu2-3112 his3-200 gal4Δ met− gal80Δ URA3:: GAL1UAS-GAL1TATA-lacZ | Clontech |
| E. coli Rosetta(DE3) | F− ompT hsdSB(rB− mB−) gal dcm (DE3) pRARE (Camr) | Novagen |
a
CFP, cyan fluorescent protein; UAS, upstream activation sequence.
b
The strain identification number is shown in parentheses.